ANTIBIOTIC SUSCEPTIBILITY OF ESCHERICHIA COLI ISOLATE FROM URINARY TRACT INFECTION OF THALASSEMIC PATIENTS IN THI-QAR PROVINCE
Escherichia coli (E. coli) is the most common type of pathogen that causes Urinary tract infection disease. It can be presented as a pathogenic or non-pathogenic strain and found not only in the animal but also in the human intestine. This bacterium can cause opportunistic infection when the human host comprised of thalassemia patients or changes the healthy hemostatic flora. This study aimed to analyze the presence of bacteria in thalassemia patients with urinary tract infection. A total of 303 samples were collected during the period from August 2019 to January 2020 from thalassemia patients who suffered from urinary tract infection. The results showed that there were 6.9% of patients infected with E. coli, 2.6% of patients were infected with S. aureus, 0.7% with both Proteus and Klebsiella, while 89.1% of patients had a negative sample for bacteria. Also, the incidence of urinary tract infections in females is higher than in males. Besides, its occurrence in rural areas is higher than in city residents. Moreover, among 16 antibiotics tested to sensitize bacteria to antibiotics, Imipenem showed 100% efficacy on all isolated bacteria. In contrast, Netilmicin showed 80.1% efficacy, Gentamycin 80.1%, and Amikacin 76.2%. Ampicillin, Aztreonam, Amoxicillin-Clavulanic Acid, Tetracycline, and Ticarcillin-Clavulanic Acid, did not show any effectiveness toward the bacteria while other antibiotics showed different activities. Furthermore, the isolated microbes from thalassemia patients were the highest resistance to antibiotics in comparison with other studies, and this antibiotic-resistant may be due to the weakening of the patient's immune status and frequent blood taking and the antibodies it contains.
Read ArticleINVESTIGATING THE EFFECT OF COLD TEMPERATURE STRESS ON UNOPENED MALE CATKINS AND INOCULATED FEMALE FLOWERS OF IRANIAN NATIVE HAZELNUT CULTIVARS
In many low-temperature areas, the environmental factor is an important limiting factor for the production and distribution of horticultural plants. This study aimed to investigate the cold tolerance of the male catkins and inoculated female flowers to screen the popular native hazelnut cultivars in Qazvin under low-temperature stress. A completely randomized factorial block design with three replications was used in this experiment with eight cultivars (Nakhnroud, Khandan, Mish-Pestan, South of Qarabagh, Asl-e-Qarabagh, Rasmi, and Gerdashkevar). After removing each of the treated samples at the end of the experiment, the samples were examined morphologically (appearance) and compared with the control. The changes were recorded as qualitative traits. To understand the influence of cold stress on reproductive organs, hydrogen peroxide and proline were measured. The results showed the onset of freezing in unopened male catkins at -7 and -9 °C and in inoculated female flowers at -3 °C. Damage to unopened male catkins' tissue occurred at -11 °C and in female flowers at -5 °C. The highest value observed among cultivars in the case for proline content of male catkins was in Mish-Pestan and Khandan cultivars with 0.816 and 0.660 µmol/ g FW, respectively. In inoculated female flowers, Mish-Pestan and Tabestaneh cultivars with 0.185 and 0.168 µmol/ g FW, respectively, showed the highest statistically significantincrease in proline content. Interestingly, the cultivars with the highest proline content in male catkins indicated the most increase in H2O2; Mish-Pestan and Khnadan with 0.569 and 0.541 ug/g FW, respectively. Asl-eQarabagh was observed to have the least H2O2 content (0.042 ug/g FW) among cultivars. Again, in inoculated female flowers, those with the highest concentration of proline (Mish-Pestan and Tabestaneh) were found to have the highest H2O2 content (0.335 and 0.331 ug/g FW, respectively
Read ArticleDENSE CLUSTERS OF RAW SEWAGE LOCATIONS ON MINNA NEIGHBOURHOOD GIS MAPS ARE POINTERS TO VERITABLE URBAN DECAY AND MOSQUITO-BREEDING GROUNDS
Background: The lack of comprehensive baseline data on raw sewage pollution in Minna, Nigeria's Niger State capital, represents a significant gap in public health information. This deficiency necessitates a systematic study to establish a database documenting sewage pollution patterns in Minna's urban environment. Aim: To employ georeferencing tools and descriptive observations for identifying and mapping potential mosquito breeding sites related to sewage discharge within Minna's built-up areas and to develop an interactive Geographic Information System (GIS) map as an environmental audit tool for public health officials. Methods: The study area was divided into five sectors: Greater Bosso, Minna Central, Greater Maitumbi, Tunga, and Greater Chanchaga. Following initial site familiarization and GIS equipment testing, systematic surveys were conducted. Field teams documented locations of household sewage discharge points, collecting geographic coordinates, temporal data (date, time, weather conditions), and site characteristics. Each identified location was photographed and recorded in standardized data sheets, including household information where available. Results: Using ArcGIS®10.8 software, comprehensive sewage pollution layers were created for each sector by integrating collected field data with Minna's township built-up and settlement shapefiles. The mapping revealed distinct pollution patterns and clusters across different neighborhoods. Discussion: Analysis of the spatial distribution showed a clear correlation between socioeconomic status and sewage management practices, with higher concentrations of improper sewage discharge in low-income areas. Conclusions: This database serves as a valuable resource for public health interventions targeting mosquito breeding grounds. Regular monitoring through periodic sewage pollution audits and expanded geographical coverage is recommended for improved urban health management.
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